Jornal de Doenças Infecciosas e Tratamento Acesso livre

Abstrato

A novel anti-CD20 monoclonal antibody shows favorable pharmacodynamic effects and acceptable safety profile in patients with multiple sclerosis

Maria Morozova

Introduction: Anti-CD20 monoclonal antibodies (mAbs) induce the B cells depletion due to Fc-mediated Antibody-Dependent Cellular Cytotoxicity
(ADCC), Complement-Dependent Cytotoxicity (CDC), or direct cells death. We developed BCD-132, a novel humanized anti-CD20 IgG1 mAb.
Materials and methods: Bio-layer interferometry, cell-based assays and aggregation stability studies proved that BCD-132 interacts with extracellular
part of CD20 antigen with nanomolar affinity. The hallmark of the new molecule is the modified Fc fragment glycosylation. The absence
of fucose determines increased affinity to the FcγRIII B cells receptors compared to rituximab (74 nM and 19.3 nM vs. 633 nM and 105 nM for
the FcγRIIIa-158F and FcγRIIIa-158V receptors, respectively, BCD-132 vs. rituximab), leads to pronounced ADCC effect. Biological activity was
established in Macaca fascicularis model of autoimmune encephalomyelitis which also showed satisfactory drug toxicity and immunogenicity.
The phase 1 (3+3 design) clinical trial sequentially included 24 patients with Remitting Multiple Sclerosis (RMS). In each of 4 cohorts participants
received the assigned dose of BCD-132 (100, 250, 500 or 1000 mg) as one or two (14-day interval) intravenous infusions. Results: We observed
the rapid depletion of CD19+/CD20+ cells within 12 hours after the BCD-132 infusion. At day 14 the median of the CD19+ cells was 0%. There
was a slight repopulation of the cells pool after 6 months (0 or <5% from baseline in BCD-132 500 mg and 1000 mg groups). There was no significant
change in the T-cells (CD3+) count. Adverse events (AEs) were not dose dependent. Severe AEs were registered in 2 (8.3%) patients (grade
3 neutropenia). Infusion-related reactions (grades 1-2) were registered in 6 (25%) patients, lasted 24 hours or less. Conclusion: BCD-132 has the
expected dose-dependent pharmacodynamic effect, in all studied doses mediates the long-term depletion of CD19+/CD20+ B lymphocytes and
possesses the acceptable safety profile in RMS patients.