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Confirmation of seeds polysaccharide structure from Cassia alata Linn. plant by periodate oxidation method

R. B. Singh

Periodate oxidation is one of the most important reaction in structural study of non-ionic polysaccharide. Periodate oxidation was done using sodium metaperiodate as oxidant proposed by Fluery & Lange’s method. It consumed 1.16 moles of periodate and liberated 0.23 moles of formic acid per mole of anhydrohexose unit after 50 hrs. The isolated water soluble seeds extract yielded sugars as D-galactose and D-mannose in 2:3 molar ratio. The probable polysaccharide structure from Cassia alata Linn. seeds have been elucidated with methylation results was confirmed by the periodate oxidation results