Jornal de Epigenética Clínica Acesso livre

Abstrato

Emergence of novel CpG islands and genomic imprinting in mammalian evolution

Shunsuke Suzuki

Introduction:

Genomic imprinting is an epigenetic regulatory mechanism which induces parent of origin dependent expression to a subset of genes. In higher vertebrates, genomic imprinting has been observed only in viviparous mammals (eutherians and marsupials) and some imprinted genes have essential roles associated with fetal and placental development and control of post natal care as well as lactation, suggesting the correlation between the evolution of genomic imprinting and these characteristics of mammals. Therefore, to study how imprinted loci arose during mammalian evolution would be of great importance to understand how these mammalian traits evolved. Parent of origin dependent expression of imprinted genes is mostly controlled by parental allele-specific DNA methylation of the CpG islands called differentially methylated regions (DMRs). Although the essential role of DMRs for genomic imprinting mechanism has been well established, little is known about how they evolved. Comparative genome analysis of the SGCE-PEG10 imprinted domain revealed that PEG10, a retro transposon-derived imprinted gene essential for placental development, was acquired in the common ancestor of marsupials and eutherians. Furthermore, in marsupials, both imprinting and differential DNA methylation were restricted to PEG10 unlike eutherians, suggesting that the insertion of PEG10 was the origin of imprinting in this imprinted domain. Also, comparative genome analyses in other imprinted domains showed that most DMRs have emerged as novel CpG islands during mammalian evolution. We presume that the emergence of novel CpG island consequent of retro transposon insertion was key genomic change for the acquisition of DMRs that evolved imprinted loci in mammalian genomes.

Recent Publications 1. Mori S, Hayashi M, Inagaki S, Oshima T, Tateishi K, Fujii H and Suzuki S (2016) Identification of multiple forms of RNA transcripts associated with human-specific retrotransposed gene copies. Genome Biology and Evolution 8(8):2288-2296. 2. Suzuki S, Shaw G and Renfree M B (2013) Postnatal epigenetic reprogramming in the germline of a marsupial, the tammar wallaby. Epigenetics & Chromatin 6(1):14. 3. Renfree M B, Suzuki S and Kaneko-Ishino T (2013) The origin and evolution of genomic imprinting and viviparity in mammals. Philosophical Transactions of the Royal Society B: Biological Sciences 368(1609):20120151. 4. Suzuki S, Shaw G, Kaneko Ishino T, Ishino F and Renfree M B (2011) The origin and evolution of genomic imprinting and viviparity in mammals. Genome Biology and Evolution 3:1276-1283. 5. Suzuki S, Ono R, Narita T, Pask A J, Shaw G, et al. (2007) Retro transposon silencing by DNA methylation can drive mammalian genomic imprinting. PLOS Genetics 3(4):e55.

Biography: Shunsuke Suzuki completed his PhD from Tokyo Institute of Technology in 2006 and Postdoctoral Studies at Tokyo Medical and Dental University during 2006–2009 and The University of Melbourne, Australia during 2009–2012. He is now an Assistant Professor at the Department of Agriculture and Life Sciences, Shinshu University, Japan. His work is focused on the role of retro transposons in the evolution of gene regulatory mechanisms in mammals.