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Micropropagation-an in vitro technique for the conservation of Alpinia galanga

Nongmaithem M. Singh, Lukram A. Chanu, Yendrembam P. Devi, Wahengbam R.C. Singh and Heigrujam B. Singh

This study was conducted to develop an efficient protocol for mass propagation of Alpinia galanga L. Explants from rhizome buds were cultured on Murashige and Skoog (MS) medium supplemented with 6-Benzylaminopurine (BAP) alone (0 to 5 mg/l) or a combination of BAP (0 to 5 mg/l) and indole 3-acetic acid (IAA) (0 to 2 mg/l). MS medium supplemented with a combination of 5.0 mg/l BAP and 2.0 mg/l IAA or 3.0 mg/l BAP and 0.5 mg/l IAA produced the highest mean number of shoots per explant as compared to other concentrations. The best shoot length was obtained on the medium containing 1.0 mg/l of BAP and 2.0 mg/l IAA. Thus, combined effects of BAP and IAA improved significantly the shoot growth and proliferation. MS medium supplemented with a combination of 5.0 mg/l BAP and 2 mg/l IAA gave the highest number of roots. However, longest roots per explant were obtained with 1.0 mg/l BAP alone. The proliferated shoots were green and healthy in appearance. Finally, healthy and complete plants with well developed roots were hardened, acclimatized and planted in the field successfully with a survival rate of 80%.