Jornal Internacional de Ciências Aplicadas - Pesquisa e Revisão Acesso livre

Abstrato

Quick Protein Expression Studies with an Automated Multi-Scale Cascade of Parallel Stirred-Tank Bioreactors

Swapna Sanika

Automation, parallelization and independent operation of trendy lab equipment, generally implemented for guide bioprocess improvement, is taken into consideration as the important thing for discount of bioprocess improvement time and costs. An automatic bioreactor gadget with four stirred-tank bioreactors on an L-scale became mixed with a personalized biomass switch gadget to distribute the mobileular suspensions produced at the L-scale into forty eight parallel stirred-tank bioreactors on an mL-scale. Afterwards parallel protein expression research automatic via way of means of a liquid managing gadget with included fluorescence reader had been performed. Isopropyl β-D1-thiogalactopyranoside-induced (IPTG) expression of the purple fluorescence protein mCherry became studied for example of the usage of fed-batch strategies with recombinant Escherichia coli.