Jetinder Kumar Kanwar and Surinder Kumar
Dianthus caryophyllus L. cv. `Tempo’ is an important cut flower in the world. We used leaves as target tissue for genetic transformation. The efficiency of two regeneration systems with insect resistance (cry 1AB) gene was compared, through Agrobacterium tumefaciens mediated transformation. 100 mg/l kanamycin was selected for transformation. The transformation frequency was greatly affected by pre-conditioning and co-cultivation duration. The highest transformation frequency17.67% was obtained using callus regeneration system. The presence and expression of the foreign gene was confirmed by PCR and insect toxicity bioassay. The selected transformants showed normal phenotypes under in vitro and in vivo conditions.
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