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Study of the volatile compounds in Artemisia absinthium from Iran using HS/SPME/GC/MS

A. Nezhadali, M. Parsa

The aerial parts of the plant Artemisia absinthium were collected in July 2010 from Binaloud mountain (Khorasan Razavi Province of Iran ). It is air dried in a shadow place. A headspace solid-phase micro-extraction (HS-SPME) method followed by gas chromatography–mass spectrometry (GC/MS) was used for the analysis of volatile compounds in the leaves of Artemisia absinthium. The dried plant was powdered and the aroma compounds of a 0.05g were isolated by HS-SPME fiber in 15 min. The chemical compounds of the plant were investigated by gas chromatography mass spectrometry (GC/MS). A total of 72 constituents, representing more than 97% of the volatiles were identified. The main compounds, were camphor (14.83%) , p-cymene (10.35%), isoledene (8.52%), caryophyllene (6.92%), isopulegol acetate (6.09%), hysterol (5.64%), isocaryophillene (5.53%), diisoamylene (5.09%), β-farnesene (3.94%) and cyclohexane,2,4 -diisopropyl-1,1 -dimethyl (3.07%). This herbal medicine traditionally uses as anthelmintic, antibacterial, antifungal, insect repellent, narcotic and digestive. Consequently, this fast and simple method can be used for the analysis of the volatile compounds emitted from Artemisia absinthium.