Avanços na Pesquisa em Ciências Aplicadas Acesso livre


Whole cell based disposable biosensor for Cadmium detection in milk

Neelam Verma Sachin Kumar and Hardeep Kaur

In the current work authors report the development of a biosensor for the detection of cadmium and its application in milk. Bacillus badius cells were immobilized onto the nylon membranes using sol-gel approach with alcohol and TEOS. The cells were mixed with sol-gel and poured onto nylon membrane and then dried. Hydrosol-gel immobilized cells were used as a biocomponent for the study. Ammonium ISE used as transducer with bioassay principle based on inhibition of urease activity. The lowest detection limit achieved was 1.0 μg/l of Cd. The prepared biosensor was applied to natural and fortified, acid extracted milk samples. The linear range of detection is between 10μg–1mg/l for Cd. Storage stability of the developed biosensor is 65 days when stored at 40C in 10% glycerol. A good correlation of results was obtained with spiked samples.