Jornal Europeu de Biologia Experimental Acesso livre

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A visionary in-vitro analysis for anti-bacterial potential using seeds of a deciduous shrub Punica granatum

Hemant Kr. Sharma, Anjali Sharma, Yunus Parvez, Harshit K. Shrivastava and Shashank Kr. Singh

Punica granatum i.e., pomegranate plays a very important role in Indian fruit market. From very ancient times pomegranate has been used for many medicinal purposes to cure many short term as well as long term disorders. The current study proves that Punica granatum is having a very strong anti-bacterial activity. For the present study firstly, we have used granatum seeds, pericarp (peel) as well as juice to evaluate the antibacterial activity of the fruits against enteric bacteria i.e. E. coli. For the detection of antibacterial activity agar gel diffusion method was used. As a result zone of inhibition was only shown by seed. On the other hand, zone of inhibition was not observed in juice and peel. So it was concluded that, the granatum seeds persists the anti-bacterial property. Secondly, solvent extract of the pomegranate peel i.e. water, ethanol and methanol extracts were prepared and antibacterial activity was tested against enteric bacteria E. coli by the method of agar diffusion method. As a result, methanol and ethanol extract showed almost same zone of inhibition (21 mm) while water extract showed comparatively less zone of inhibition (13 mm) with respect to control ofloxacin (32 mm) at 150 ppm.