Jornal Europeu de Biologia Experimental Acesso livre

Abstrato

Comparison of three different DNA extraction methods from positive smears prepared from lesions of patients with cutaneous leishmaniasis

Gh. R. Farnoosh, K. Hassanpour, R. A. Taheri, M. Ghamgosha, M. R. Mahmoudian Sani and M. Mellat

Cutaneous leishmaniasis is a dermatic parasitical infection caused by a vector-borne pathogenic patasite.The best and simplest identification method is sampling from lesion borders and taking tissue containing leishmania and prepared smear-positive smesrs are used for DNA extraction. Since DNA extraction is an important stage in molecular diagnostic test, obtaining favorable DNA requires a sensitive and easy as well as cost effective method .The aim of this study was comparing different extraction methods for identisication of different leishmania species. Our results show that although boiling method, despite simplicity and cheapness cannot be used for DNA extraction from smears because of its poor quality. The phenol-chloroform based methods are as valuable as DNeasy mini kit (Kiagene) but more cost effective than kit.The advantages of DNeasy mini kit are simplicity and less time consuming compare with phenol - chloroform based methods.