Jornal Europeu de Biologia Experimental Acesso livre

Abstrato

DNA fingerprinting and CRISPR cas9 system

Umair Masood

Leicester university geneticist Alec Jeffrey’s develops a technique called DNA finger printing in 1985 it allows the DNA sample from different people to be compared look for similarities and differences. It used the solving crime and can confirm if the people are related to each other like paternity testing. There is section of chromosomes where an instead of gene consisting of a long sequence of bases, they are  usually 15-100 base pairs long that are repeated in many times these repeated sequences called Variable Number of Tandem Repeat. we can know the VNTR sequence of any person than we can design a gRNA. Let's say you have a DNA sample with fluorescent labeled from victim and you want to make sure that VNTR sequence you are interested is in match with victim. We can design a CRISPR to scan through DNA or find specific VNTR. The CRISPR scan the DNA if the CRISPR does not find targeted VNTR it does not bind to it its means that no fluorescence color appears under UV-light but its scan and find its target and this binding create a fluorescence signal its means that VNTR can be occur in a  DNA