Jornal Europeu de Biologia Experimental Acesso livre

Abstrato

The role of cytokines, TNF-α, IL-6 and pregnancy associated hormones in Toxoplasma gondii induced abortion

Waleed K. El-Hashimi, Hutham I. Khalila, Qasim S. Al-Mayah and Nada M. Al-Bashier

Toxoplasma gondii is a protozoan that can cross the placenta of the infected mother and causes severe fetal damage or abortion. This study aimed to investigate the role of two cytokines and sex hormones in the T. gondiiinduced abortion. A total of 135 placentas from spontaneously aborted women and 13 from women with induced abortion were underwent immunohistochemical test for T. gondii antigen. Out of the 135 women, 34 gave positive result. Accordingly, the study population were divided into three groups: aborted women positive for T. gondii, aborted women negative for T. gondii, and women with induced abortion. TNF-α and IL-6 intensity in placental sections were determined by IHC. Enzyme-linked immunosorbent assay (ELISA) was used to estimate serum level of progesterone and estradiol. Aborted women positive for T. gondii had significantly higher percentage of trophoblasts expressing TNF-α and IL-6 than both aborted women negative for T. gondii and women with induced abortion. Whereas, women with induced abortion demonstrated higher serum level of estradiol and progesterone than aborted women positive or negative for T. gondii.